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KMID : 1094020080250040268
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Journal of Veterinary Clinics
2008 Volume.25 No. 4 p.268 ~ p.273
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Production of High Quality Pork with Feed Additives of Lacquer Tree Extracts and Development of Meat Processing Technique V. Lymphocyte Distribution and Morphological Change in Small Intestine of Pigs after Fed with Fermented Lacquer Tree Extracts
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Kim Sang-Hyun
Cho Kyu-Woan
Choi Chung-Kuk
Seuong Nak-Ju
Park Joong-Choon
Song Young-Min
Cha Hye-Jin
Kim Yong-Hwan
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Abstract
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For evaluation of immune stimulation effect of lacquer tree extracts, lymphocytes were counted by labeling of FITC-conjugated monoclonal antibody in the pheripheral blood of pigs that fed with a fodder supplemented by lacquer tree extracts. Populations of MHC-II+, CD4+, and CD8+T lymphocytes were increased more than 2% level after 1 week feed supply of the lacquer tree extracts. The increase of those T cells reached at maximum level after 2 weeks in the tested group. B lympyocytes with surface IgM were increased 5% after 1 week feed supply of the lacquer tree extracts, and their numbers reached maximum after 2 weeks in the tested group. For the assessment of cytotoxicity of the lacquer tree extracts, morphological changes were examined on the epithelial cells of small intestine from pigs fed with a fodder supplemented by 0.1 % lacquer tree extracts for 6 weeks (the tested group). Thin-sectioned tissue of small intestine was fixed with glutaraldehyde, then coated with gold particles, and the specimen was examined under scanning electron microscope. The villi on the mucus membrane of jejunum and ileum from the tested pigswere enlarged on the tip and were linked each other.
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KEYWORD
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lacquer tree extracts, lymphocyte differentiation, surface IgM
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